Antigenicity analysis of different regions of the severe acute respiratory syndrome coronavirus nucleocapsid protein
Identifieur interne : 005B73 ( Main/Exploration ); précédent : 005B72; suivant : 005B74Antigenicity analysis of different regions of the severe acute respiratory syndrome coronavirus nucleocapsid protein
Auteurs : ZELIANG CHEN [République populaire de Chine] ; DECUI PEI [République populaire de Chine] ; LINGXIAO JIANG [République populaire de Chine] ; YAJUN SONG [République populaire de Chine] ; JIN WANG [République populaire de Chine] ; HONGXIA WANG [République populaire de Chine] ; DONGSHENG ZHOU [République populaire de Chine] ; JUNHUI ZHAI [République populaire de Chine] ; ZONGMIN DU [République populaire de Chine] ; BEI LI [République populaire de Chine] ; MAOFENG QIU [République populaire de Chine] ; YANPING HAN [République populaire de Chine] ; ZHAOBIAO GUO [République populaire de Chine] ; RUIFU YANG [République populaire de Chine]Source :
- Clinical chemistry : (Baltimore, Md.) [ 0009-9147 ] ; 2004.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Biochimie.
English descriptors
- KwdEn :
Abstract
Background: The widespread threat of severe acute respiratory syndrome (SARS) to human health has made urgent the development of fast and accurate analytical methods for its early diagnosis and a safe and efficient antiviral vaccine for preventive use. For this purpose, we investigated the antigenicity of different regions of the SARS coronavirus (SARS-CoV) nucleocapsid (N) protein. Methods: The cDNA for full-length N protein and its various regions from the SARS-CoV was cloned and expressed in Escherichia coli. After purification, all of the protein fragments were printed on glass slides to fabricate a protein microarray and then probed with the sera from SARS patients to determine the reactivity of these protein fragments. Results: The full-length protein and two other fragments reacted with all 52 sera tested. Four important regions with possible epitopes were identified and named as EP1 (amino acids 51-71), EP2 (134-208), EP3 (249-273), and EP4 (349-422), respectively. EP2 and EP4 possessed linear epitopes, whereas EP1 and EP2 were able to form conformational epitopes that could react with most (>80%) of the tested sera. EP3 and EP4 also formed conformational epitopes, and antibodies against these epitopes existed in all 52 of the sera tested. Conclusion: The N protein is a highly immunogenic protein of the SARS-CoV. Conformational epitopes are important for this protein, and antigenicity of the COOH terminus is higher than that of the NH2 terminus. The N protein is a potential diagnostic antigen and vaccine candidate for SARS-CoV.
Affiliations:
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type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Decui Pei" sort="Decui Pei" uniqKey="Decui Pei" last="Decui Pei">DECUI PEI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute af Microbiology and Epidemiology, Academy of Military Medical Sciences</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lingxiao Jiang" sort="Lingxiao Jiang" uniqKey="Lingxiao Jiang" last="Lingxiao Jiang">LINGXIAO JIANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute af Microbiology and Epidemiology, Academy of Military Medical Sciences</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yajun Song" sort="Yajun Song" uniqKey="Yajun Song" last="Yajun Song">YAJUN SONG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute af Microbiology and Epidemiology, Academy of Military Medical Sciences</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Jin Wang" sort="Jin Wang" uniqKey="Jin Wang" last="Jin Wang">JIN WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute af Microbiology and Epidemiology, Academy of Military Medical Sciences</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Hongxia Wang" sort="Hongxia Wang" uniqKey="Hongxia Wang" last="Hongxia Wang">HONGXIA WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute af Microbiology and Epidemiology, Academy of Military Medical Sciences</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>1 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type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Junhui Zhai" sort="Junhui Zhai" uniqKey="Junhui Zhai" last="Junhui Zhai">JUNHUI ZHAI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute af Microbiology and Epidemiology, Academy of Military Medical Sciences</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zongmin Du" sort="Zongmin Du" uniqKey="Zongmin Du" last="Zongmin Du">ZONGMIN DU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute af Microbiology and Epidemiology, Academy of Military Medical Sciences</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Bei Li" sort="Bei Li" uniqKey="Bei Li" last="Bei Li">BEI LI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute af Microbiology and Epidemiology, Academy of Military Medical Sciences</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Maofeng Qiu" sort="Maofeng Qiu" uniqKey="Maofeng Qiu" last="Maofeng Qiu">MAOFENG QIU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute af Microbiology and Epidemiology, Academy of Military Medical Sciences</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yanping Han" sort="Yanping Han" uniqKey="Yanping Han" last="Yanping Han">YANPING HAN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute af Microbiology and Epidemiology, Academy of Military Medical Sciences</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhaobiao Guo" sort="Zhaobiao Guo" uniqKey="Zhaobiao Guo" last="Zhaobiao Guo">ZHAOBIAO GUO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute af Microbiology and Epidemiology, Academy of Military Medical Sciences</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ruifu Yang" sort="Ruifu Yang" uniqKey="Ruifu Yang" last="Ruifu Yang">RUIFU YANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute af Microbiology and Epidemiology, Academy of Military Medical Sciences</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title><title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title><idno type="ISSN">0009-9147</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title><title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title><idno type="ISSN">0009-9147</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigenicity</term><term>Biochemical analysis</term><term>Biochemistry</term><term>Clinical biology</term><term>Coronavirus</term><term>Molecular biology</term><term>Nucleocapsid</term><term>Protein</term><term>Severe acute respiratory syndrome</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Antigénicité</term><term>Analyse biochimique</term><term>Syndrome respiratoire aigu sévère</term><term>Coronavirus</term><term>Nucléocapside</term><term>Protéine</term><term>Biochimie</term><term>Biologie clinique</term><term>Biologie moléculaire</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Biochimie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: The widespread threat of severe acute respiratory syndrome (SARS) to human health has made urgent the development of fast and accurate analytical methods for its early diagnosis and a safe and efficient antiviral vaccine for preventive use. For this purpose, we investigated the antigenicity of different regions of the SARS coronavirus (SARS-CoV) nucleocapsid (N) protein. Methods: The cDNA for full-length N protein and its various regions from the SARS-CoV was cloned and expressed in Escherichia coli. After purification, all of the protein fragments were printed on glass slides to fabricate a protein microarray and then probed with the sera from SARS patients to determine the reactivity of these protein fragments. Results: The full-length protein and two other fragments reacted with all 52 sera tested. Four important regions with possible epitopes were identified and named as EP1 (amino acids 51-71), EP2 (134-208), EP3 (249-273), and EP4 (349-422), respectively. EP2 and EP4 possessed linear epitopes, whereas EP1 and EP2 were able to form conformational epitopes that could react with most (>80%) of the tested sera. EP3 and EP4 also formed conformational epitopes, and antibodies against these epitopes existed in all 52 of the sera tested. Conclusion: The N protein is a highly immunogenic protein of the SARS-CoV. Conformational epitopes are important for this protein, and antigenicity of the COOH terminus is higher than that of the NH<sub>2</sub> terminus. The N protein is a potential diagnostic antigen and vaccine candidate for SARS-CoV.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zeliang Chen" sort="Zeliang Chen" uniqKey="Zeliang Chen" last="Zeliang Chen">ZELIANG CHEN</name></noRegion><name sortKey="Bei Li" sort="Bei Li" uniqKey="Bei Li" last="Bei Li">BEI LI</name><name sortKey="Decui Pei" sort="Decui Pei" uniqKey="Decui Pei" last="Decui Pei">DECUI PEI</name><name sortKey="Dongsheng Zhou" sort="Dongsheng Zhou" uniqKey="Dongsheng Zhou" last="Dongsheng Zhou">DONGSHENG ZHOU</name><name sortKey="Hongxia Wang" sort="Hongxia Wang" uniqKey="Hongxia Wang" last="Hongxia Wang">HONGXIA WANG</name><name sortKey="Jin Wang" sort="Jin Wang" uniqKey="Jin Wang" last="Jin Wang">JIN WANG</name><name sortKey="Junhui Zhai" sort="Junhui Zhai" uniqKey="Junhui Zhai" last="Junhui Zhai">JUNHUI ZHAI</name><name sortKey="Lingxiao Jiang" sort="Lingxiao Jiang" uniqKey="Lingxiao Jiang" last="Lingxiao Jiang">LINGXIAO JIANG</name><name sortKey="Maofeng Qiu" sort="Maofeng Qiu" uniqKey="Maofeng Qiu" last="Maofeng Qiu">MAOFENG QIU</name><name sortKey="Ruifu Yang" sort="Ruifu Yang" uniqKey="Ruifu Yang" last="Ruifu Yang">RUIFU YANG</name><name sortKey="Yajun Song" sort="Yajun Song" uniqKey="Yajun Song" last="Yajun Song">YAJUN SONG</name><name sortKey="Yanping Han" sort="Yanping Han" uniqKey="Yanping Han" last="Yanping Han">YANPING HAN</name><name sortKey="Zhaobiao Guo" sort="Zhaobiao Guo" uniqKey="Zhaobiao Guo" last="Zhaobiao Guo">ZHAOBIAO GUO</name><name sortKey="Zongmin Du" sort="Zongmin Du" uniqKey="Zongmin Du" last="Zongmin Du">ZONGMIN DU</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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